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JoVE Journal Neuroscience
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
 

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Article DOI: 10.3791/4141-v • 09:42 min • August 17th, 2012
August 17th, 2012 •
1, 1,2
1Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

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An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

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Genetic Study Axon Regeneration Cultured Adult Dorsal Root Ganglion Neurons Central Nervous System (CNS) Peripheral Nervous System (PNS) Mature Neurons Axon Growth Hostile Environment Peripheral Nerve Injuries Conditioning Lesion Spinal Cord Injury Molecular Mechanisms CNS Axon Regeneration
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