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Reverse genetikk Mediated Gjenvinning av Infeksiøs murin Norovirus
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Capitoli
- 00:05Titolo
- 01:34Procedure Overview
- 03:16RNA Transcription and Capping for the Recovery of Infectious MNV
- 06:27MNV Recovery by RNA Electroporation into Raw Cells
- 08:25Recovery by Lipofection into BSR-T7 Cells
- 10:07Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase
- 12:20Representative Results
Noroviruses are a major cause of gastroenteritis yet molecular techniques for their characterisation are still relatively new. Here we report two different reverse genetics approaches for the efficient recovery of murine norovirus (MNV), the only member of this genus which can be propagated in cell culture.
