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Capitoli
- 00:05Titolo
- 03:04Substrate Preparation and Cell Culture
- 04:30Fabrication of PDMS VMMC
- 06:37Cell Shearing
- 08:30Monocyte Adhesion Assay
- 10:55Representative Results
- 11:27Conclusion
Microfluidic प्रवाह photolithography द्वारा etched और PDMS से निर्मित कक्षों कार्यात्मक चुनाव आयोग शिथिलता और सूजन के साथ जुड़े परिणामों की जांच करने के लिए लागू कर रहे हैं. एक प्रतिनिधि प्रयोग में, अंतर कतरनी तनाव की क्षमता cytokine सक्रिय ईसी monolayers monocytic कोशिका आसंजन मिलाना प्रदर्शन किया है.
Tags
On-Chip Endothelial Inflammatory PhenotypingAtherogenesisMetabolic AbnormalitiesSystemic InflammationEndothelial DysfunctionFunctional Changes In EndotheliumRisk AssessmentTherapeutic StrategyRegulation Of InflammationLocal HemodynamicsNon-random Spatial Distribution Of AtherosclerosisLab-on-a-chipQuantitatively Assay Metabolic PerturbationInflammatory Events In Endothelial CellsMonocytesFlow ConditionsSoft LithographyMicrofabricate Vascular Mimetic Microfluidic Chambers (VMMC)Cultured EC MonolayersImaging Inflammatory EventsShear FieldCytokine-induced InflammationLipid-induced InflammationRAGE-induced InflammationHuman Aortic EC (HAEC)Monocytic Cell Rolling And ArrestTNF-alpha
