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A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field
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Neuroscienze
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JoVE Journal Neuroscienze
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

DOI:
10.3791/4193-v

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11:00 min

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October 13, 2012

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, ,
1Institute for Biomaterials and Biomedical Engineering,University of Toronto, 2Lyndhurst Centre,Toronto Rehabilitation Institute, 3Department of Surgery,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:25Galvanotaxis Chamber Preparation
  • 05:30Live Cell Time-lapse Imaging
  • 08:55Kinematic and IHC Analysis of Differentiated and Undifferentiated NPCs
  • 10:35Conclusion

Summary

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In this protocol we demonstrate how to construct custom chambers that permit the application of a direct current electric field to enable time-lapse imaging of adult brain derived neural precursor cell translocation during galvanotaxis.

Tags

Galvanotaxis AssayNeural Precursor Cell MigrationExternally Applied Direct Current Electric FieldNeural Stem CellsNeuroregenerative StrategiesMobilization Of NPCsLesion SiteEndogenous PrecursorsPeriventricular RegionMechanisms Of NPC MigrationDirect Current Electric Fields (dcEFs)Galvanotaxis PhenomenonPhysiological Electric FieldsCell MigrationNormal DevelopmentWound Repair

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