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细菌毒素引起的响应，用活细胞荧光显微镜的可视化
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
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细菌毒素引起的响应，用活细胞荧光显微镜的可视化

DOI:
10.3791/4227-v

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14:29 min

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October 01, 2012

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, , ,
1Department of Immunology,University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

Capitoli

  • 00:05Titolo
  • 01:09Preparation of Streptolysin O
  • 05:22Cell Lytic Assay
  • 06:44Micropipette Delivery of Toxin to Cultured Cells
  • 10:24Representative Purification and Imaging Results
  • 13:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

方法从重组净化胆固醇结合毒素链球菌溶血素O<em> E.大肠杆菌</em>和可视化的生活真核细胞毒素结合进行了阐述。本地化的交付毒素诱导靶细胞，揭示新的毒素生物学方面的快速而复杂的变化。

Tags

VisualizationBacterial ToxinLive Cell Fluorescence MicroscopyMembranePoresLeakageCellular ContentsInfluxToxin ExposureCell TypeInflammatory ResponsesImmune CellsMacrophagesPro-inflammatory CytokinesGram Positive BacteriaVirulence MechanismsMorphologic ChangesPlasma MembraneCholesterol-enriched Surface ProtrusionsExtracellular SpaceCellular Defense MechanismEM (electron Microscopy)Chemical FixationImmune CellsMacrophagesInflammationMembrane VesiclesIL-1 Family Cytokines

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