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Neural Eksplantasjon kulturer fra<em> Xenopus laevis</em
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JoVE Journal Neuroscienze
Neural Explant Cultures from Xenopus laevis

Neural Eksplantasjon kulturer fra<em> Xenopus laevis</em

DOI:
10.3791/4232-v

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10:05 min

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October 15, 2012

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, , ,
1Department of Cell Biology,Harvard Medical School

Capitoli

  • 00:05Titolo
  • 00:57Introduction
  • 01:14In vitro Fertilization of Xenopus Eggs
  • 02:34Microinjection of RNA
  • 04:46Neural Tube Dissection and Plating
  • 09:08Results: Imaging Growth Cones from Xenopus Neuronal Explants
  • 09:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

Dyrking nevrale explants fra dissekert<em> Xenopus laevis</em> Embryo som uttrykker fluorescerende fusjonsproteiner tillater avbildning av vekst cone cytoskeletal dynamikk.

Tags

Neural Explant CulturesXenopus LaevisAxon GuidanceGrowth ConeGuidance CuesCytoskeleton ModulationCytoskeletal Protein DynamicsEffector DynamicsHigh-resolution Live ImagingDevelopmental Neurobiology StudiesGrowth Cone Microtubule DynamicsFluorescently Tagged Cytoskeletal Fusion ProteinsGene Expression ManipulationNeural Tube StageOutgrowing Neurites

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