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糖基谱研究植物细胞壁聚合物，用微阵列
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Biologia
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JoVE Journal Biologia
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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糖基谱研究植物细胞壁聚合物，用微阵列

DOI:
10.3791/4238-v

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12:30 min

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December 17, 2012

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, , , , ,
1Australian Centre of Excellence in Plant Cell Walls, School of Botany,University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany,University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology,University of Copenhagen

Capitoli

  • 00:05Titolo
  • 01:40Tissue Collection & Preparation
  • 03:01Extraction of Cell Wall Glycans
  • 04:20Printing Microarrays
  • 05:45Probing of Glycan Microarrays
  • 06:36Quantification
  • 07:49Results: CoMPP Profiling of N. alata
  • 11:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

一种称为<strong> C</strong> omprehensive<strong> M</strong> icroarray<strong> P</strong> olymer探查（CoMPP）植物细胞壁聚糖的特性进行说明。该方法结合了与一个微型芯片分析平台允许筛选聚糖发生在一个广泛的范围内的生物上下文定义的聚糖-抗原决定簇特异性的单克隆抗体。

Tags

Glycan ProfilingPlant Cell Wall PolymersMicroarraysHeterogeneous GlycansBiosynthesisFunctionGlycosyl ResiduesLinkagesRing StructureIsomeric ConfigurationAnomeric ConfigurationNon-sugar ResiduesDevelopmentEnvironmental CuesGenesArabidopsisReverse Genetics Approaches

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