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的斑马鱼胚胎神经上皮通透性的测定
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Neuroscienze
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JoVE Journal Neuroscienze
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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的斑马鱼胚胎神经上皮通透性的测定

DOI:
10.3791/4242-v

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06:37 min

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October 24, 2012

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,
1Department of Biology,Massachusetts Institute of Technology, 2Whitehead Institute of Biomedical Research

Capitoli

  • 00:05Titolo
  • 01:05Preparing Injection Needles and Embryos
  • 02:22Injecting Brain Ventricles
  • 03:22Image and Quantification of Dye Movement
  • 04:44Representative Permeability Results of Neuroepithelium
  • 06:18Conclusion

Summary

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我们描述了现场的整个动物的胚胎斑马鱼脑通透性的定量测量。该技术分析神经管的管腔内保留脑脊液和不同分子量的分子的能力，和它们的运动量化满分心室。此方法是非常有用的用于确定在上皮通透性和成熟发展和疾病过程中的差异。

Tags

PermeabilityZebrafish Embryonic NeuroepitheliumBrain Ventricular SystemBrain VentriclesCerebrospinal Fluid (CSF)Protein-rich FluidNeural Tube FormationClaudin 5aNaK-ATPaseTight Junction FormationVentricular Dye Retention AssayMyosin Inhibition

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