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Immunologia e infezioni

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通过Bioorthogonal点击化学化学选择性病毒表面改性

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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

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通过Bioorthogonal点击化学化学选择性病毒表面改性

DOI:

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12:31 min

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August 19, 2012

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Frederick A. Rubino, Yoon Hyeun Oum, Lakshmi Rajaram, Yanjie Chu, Isaac S. Carrico

¹Department of Chemistry,Stony Brook University

Capitoli

00:00Titolo
02:04Production of Aha-Labeled Adenovirus
04:28Production of Azido-Sugar-Labeled Adenovirus
05:46Purification of Azide-Labeled Adenoviral Particles
07:34Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
08:32Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
09:32SDS-PAGE Analysis of Clicked Adenovirus
10:34Conclusion

Summary

Traduzione automatica

腺病毒颗粒设计，含有非天然氨基酸类似物azidohomoalanine或叠氮糖<em>Ø</em> GlcNAz。每叠组chemoselectively结扎通过“点击”化学反应病毒表面改性的一种手段。

Tags

Chemoselective Modification Viral Surfaces Bioorthogonal Click Chemistry Gene Therapy Oncolytic Applications Vaccine Development Genetics-based Approaches Chemoselective Ligation Targeting Molecules Dyes Azide Tags Metabolic Labeling Effector Ligands Adenovirus Particles Azidohomoalanine O-linked N-azidoacetylglucosamine

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