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病毒遗传背景明确的神经回路跟踪

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Viral Tracing of Genetically Defined Neural Circuitry

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病毒遗传背景明确的神经回路跟踪

DOI:

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13:06 min

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October 17, 2012

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Kevin Beier, Constance Cepko

¹Department of Genetics,Harvard Medical School, ²Howard Hughes Medical Institute,Harvard Medical School

Capitoli

00:05Titolo
01:16Passage and Concentration of Vesicular Stomatitis Virus (VSV)
04:23Stereotaxic Injection of Virus
08:19Tissue Harvest and Preparation for Microscopic Analysis
09:41Results: Behavior of rVSV(A/RG) Virus in Various Cell Types
12:22Conclusion

Summary

Traduzione automatica

跟踪突触连接的神经元的方法。我们使用TVA特异性的上游电池，探测是否感兴趣的接收的细胞群体突触输入从基因定义的细胞类型。

Tags

Viral Tracing Genetically Defined Neural Circuitry Transsynaptic Viruses Pseudorabies Virus Rabies Virus Vesicular Stomatitis Virus Anterograde Direction Retrograde Direction Monosynaptic Retrograde Tracing Glycoprotein Gene Chimeric G TVA Receptor Inputs Onto A Defined Cell Type VSV As A Transsynaptic Tracer

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