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Udforskning arteriernes glatte muskulatur Kv7 kaliumkanal Funktion hjælp Patch Clamp Elektrofysiologi og Pressure Myography
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Biologia
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JoVE Journal Biologia
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography

Udforskning arteriernes glatte muskulatur Kv7 kaliumkanal Funktion hjælp Patch Clamp Elektrofysiologi og Pressure Myography

DOI:
10.3791/4263-v

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11:02 min

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September 14, 2012

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, , ,
1Department of Molecular Pharmacology & Therapeutics,Loyola University Chicago

Capitoli

  • 00:05Titolo
  • 02:08Dissection and Cleaning of Arteries
  • 03:04Cell Isolation for Patch Clamp Studies
  • 04:50Whole-cell Recording to Measure Kv7 Potassium Currents or Membrane Voltage
  • 06:36Use of Intact Artery Segments for Pressure Myography
  • 09:00Results: Kv7 Potassium Channel Function of Arterial Smooth Muscle using Patch Clamp Electrophysiology and Pressure Myography
  • 10:33Conclusion

Summary

Traduzione automatica

Traduzione automatica

Målinger af Kv7 (KCNQ) kalium kanal aktivitet i isolerede arterielle myocytter (ved hjælp af patch clamp elektrofysiologiske teknikker) parallelt med målinger af kvælerslange / dilator reaktioner (med tryk myography) kan afsløre vigtige oplysninger om de roller, Kv7 kanaler i vaskulære glatte muskulatur fysiologi og farmakologi.

Tags

Arterial Smooth MuscleKv7 Potassium ChannelPatch Clamp ElectrophysiologyPressure MyographySmooth Muscle CellsResistance ArteriesArtery DiameterBlood FlowSystemic Blood PressureCytosolic Calcium ConcentrationIon TransportersIon ChannelsSignal Transduction PathwaysVasoactive HormonesVasoconstrictionVasodilationTherapeutic AgentsCalcium Channel BlockersKv7 Voltage-activated Potassium ChannelsPhysiological Signal TransductionRat Mesenteric Arteries

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