È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 02:08Dissection and Cleaning of Arteries
- 03:04Cell Isolation for Patch Clamp Studies
- 04:50Whole-cell Recording to Measure Kv7 Potassium Currents or Membrane Voltage
- 06:36Use of Intact Artery Segments for Pressure Myography
- 09:00Results: Kv7 Potassium Channel Function of Arterial Smooth Muscle using Patch Clamp Electrophysiology and Pressure Myography
- 10:33Conclusion
Metingen van KV7 (KCNQ) kaliumkanaal activiteit in geïsoleerde bloedvaten myocyten (met behulp van patch-clamp elektrofysiologische technieken), parallel met metingen van constrictor / dilatator reacties (met behulp van druk myografie) kunnen belangrijke informatie over de rol van KV7 kanalen in het gladde spierweefsel fysiologie en farmacologie.
Tags
Arterial Smooth MuscleKv7 Potassium ChannelPatch Clamp ElectrophysiologyPressure MyographySmooth Muscle CellsResistance ArteriesArtery DiameterBlood FlowSystemic Blood PressureCytosolic Calcium ConcentrationIon TransportersIon ChannelsSignal Transduction PathwaysVasoactive HormonesVasoconstrictionVasodilationTherapeutic AgentsCalcium Channel BlockersKv7 Voltage-activated Potassium ChannelsPhysiological Signal TransductionRat Mesenteric Arteries
