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Capitoli
- 00:05Titolo
- 01:23Protein Expression and Purification
- 03:41DNA Purification
- 07:35Protein-DNA Complex Formation and Analysis
- 08:35Results: Crystal Structure of SeqA delta(41-59)-A25R Bound to Hemimethylated DNA
- 11:08Conclusion
Krystalstrukturen af protein-DNA-komplekser kan give indsigt i proteinfunktion, mekanisme, såvel som af arten af den specifikke interaktion. Her rapporterer vi hvordan man kan optimere længde, sekvens og ender duplex DNA for co-krystallisering med<em> Escherichia coli</em> SeqA, en negativ regulator af replikation indvielse.
Tags
DNA DuplexesCrystallizationSeqA-DNA ComplexesEscherichia Coli SeqANegative RegulatorDNA ReplicationPremature Reinitiation EventsHemimethylated GATC ClustersOrigin Of ReplicationReplication ForksHigher Ordered StructuresHigh Affinity ComplexesMinimal Functional UnitDimerGATC SequencesMolecular LevelStructural AnalysisProtein-DNA CrystallizationPacking InteractionsProtein-protein Interactions
