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Tissue Engineering of the Intestine in a Murine Model
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JoVE Journal Bioingegneria
Tissue Engineering of the Intestine in a Murine Model

Tissue Engineering of the Intestine in a Murine Model

DOI:
10.3791/4279-v

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08:45 min

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December 01, 2012

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, , , , , , ,
1Children’s Hospital Los Angeles, Division of Pediatric Surgery, Saban Research Institute,Keck School of Medicine of the University of Southern California

Capitoli

  • 00:00Titolo
  • 00:26Section 1: Organoid Units Preparation
  • 04:32Section 2: Loading of Polyglycolic Acid Scaffold
  • 05:43Section 3: Implantation into Host Mouse
  • 07:23Section 4: Harvest

Summary

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This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.

Tags

IntestineMurine ModelTESISmall Bowel ResectionShort Bowel SyndromeFunctional Malabsorptive StateMorbidityMortalityHealthcare CostsParenteral Nutrition DependenceLiver FailureCirrhosisMultivisceral Organ TransplantationMulticellular Organoid Units-on-scaffold ApproachMucosal ElementsMesenchymal ElementsIntestinal Stem Cell NicheTransgenic ToolsImmunocompromised Mouse StrainsMouse Xenograft FormationGood Manufacturing Practice (GMP) Reagents

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