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Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
JoVE Journal
Medicina
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JoVE Journal Medicina
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer

Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer

DOI:
10.3791/4321-v

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16:59 min

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October 10, 2012

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, ,
1Department of Medicine,Baylor College of Medicine , 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center,Baylor College of Medicine , 3Department of Molecular & Cellular Biology,Baylor College of Medicine

Capitoli

  • 00:05Titolo
  • 02:00Helper-dependent Adenoviral Vector Production and Amplification
  • 05:02Large Scale HDAd Production
  • 07:13Vector Purification
  • 11:19Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
  • 13:08Evidence for Neo-islet Formation in Liver: Representative Results
  • 16:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.

Tags

Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia

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