Rivista
/
/
Genoom-brede gendeleties in<em> Streptococcus sanguinis</em> Door High Throughput PCR
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
DOI:

14:07 min

November 23, 2012

,

Capitoli

  • 00:05Titolo
  • 01:24High-throughput PCR Amplification and Purification
  • 06:24Preparation of Competent Cells, Cell Transformation and Antibiotic Selection
  • 08:30Mutant Confirmation and Storage
  • 10:34Results: Creation of Recombinant PCR Constructs and Correct Mutants
  • 13:48Conclusion

Summary

Traduzione automatica

Een efficiënte genoom-brede enkel gen mutatie methode is vastgesteld aan de hand<em> Streptococcus sanguinis</em> Als modelorganisme. Deze methode heeft bereikt via high throughput recombinant PCRs en transformaties.

Video correlati

Read Article