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Manufacturing and Using Piggy-back Multibarrel Electrodes for In vivo Pharmacological Manipulations of Neural Responses
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Manufacturing and Using Piggy-back Multibarrel Electrodes for In vivo Pharmacological Manipulations of Neural Responses
DOI:

06:52 min

January 18, 2013

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Capitoli

  • 00:05Titolo
  • 01:13Pull Glass Electrodes
  • 02:57Assemble Piggy-back Electrode
  • 04:06Fill and Prepare Electrodes
  • 04:41Results: Titration of Ejection Currents
  • 06:15Conclusion

Summary

Traduzione automatica

Iontophoresis of neural agonists and antagonists during extracellular in vivo recordings is a powerful way to manipulate a neuron’s microenvironment. These manipulations can most easily be done via piggy-back multibarrel electrodes. Here we describe how to manufacture them and use them during auditory recordings.

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