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SNARE蛋白介导的膜融合酶的细胞融合含量的分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

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SNARE蛋白介导的膜融合酶的细胞融合含量的分析

DOI:

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09:19 min

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October 19, 2012

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Nazarul Hasan, David Humphrey, Krista Riggs, Chuan Hu

¹Department of Biochemistry and Molecular Biology,University of Louisville School of Medicine

Capitoli

00:05Titolo
01:19Cell Surface SNARE Expression Analysis
03:29Enzymatic Cell Fusion Assay
06:55SNARE-mediated Interactions between SNAP-25/Syntaxin1/Syntaxin4 and VAMPs
08:46Conclusion

Summary

Traduzione automatica

我们已经开发出一种量化SNARE介导膜融合事件由激活的β-半乳糖苷酶的表达的细胞融合实验。

Tags

SNARE-mediated Membrane Fusion Enzymatic Cell Fusion Assay SNARE Proteins V-SNAREs T-SNAREs Intracellular Vesicle Fusion Reconstitution Assays Cell Fusion Assay Flipped SNARE Proteins Microscopic Analysis Quantification Of Cell Fusion Events β-galactosidase Tet-Off Gene Expression System Tetracycline-controlled Transactivator (tTA)Reporter Plasmid LacZ Gene Tetracycline-response Element (TRE-LacZ)COS-7 Cells

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