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Analyse af SNARE-medieret membranfusion anvendelse af en enzymatisk Cellefusion Assay
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Biologia
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JoVE Journal Biologia
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Analyse af SNARE-medieret membranfusion anvendelse af en enzymatisk Cellefusion Assay

DOI:
10.3791/4378-v

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09:19 min

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October 19, 2012

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, , ,
1Department of Biochemistry and Molecular Biology,University of Louisville School of Medicine

Capitoli

  • 00:05Titolo
  • 01:19Cell Surface SNARE Expression Analysis
  • 03:29Enzymatic Cell Fusion Assay
  • 06:55SNARE-mediated Interactions between SNAP-25/Syntaxin1/Syntaxin4 and VAMPs
  • 08:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi har udviklet en cellefusion assay, der kvantificerer SNARE-medierede membranfusionsteknikker begivenheder ved aktiveret ekspression af β-galactosidase.

Tags

SNARE-mediated Membrane FusionEnzymatic Cell Fusion AssaySNARE ProteinsV-SNAREsT-SNAREsIntracellular Vesicle FusionReconstitution AssaysCell Fusion AssayFlipped SNARE ProteinsMicroscopic AnalysisQuantification Of Cell Fusion Eventsβ-galactosidaseTet-Off Gene Expression SystemTetracycline-controlled Transactivator (tTA)Reporter PlasmidLacZ GeneTetracycline-response Element (TRE-LacZ)COS-7 Cells

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