Rivista
/
Biologia
/
一个高含量的成像流程研究Grb2的信号复合体的表达克隆
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
Please note that all translations are automatically generated. Click here for the English version.

一个高含量的成像流程研究Grb2的信号复合体的表达克隆

DOI:
10.3791/4382-v

•

10:52 min

•

October 30, 2012

•

, , ,
1MRC LMCB,University College London, 2Center for Computational and Integrative Biology,Massachusetts General Hospital

Capitoli

  • 00:05Titolo
  • 01:27Preparation of cDNA Expression Library
  • 05:43Cell Seeding and Transfection
  • 07:10Image Acquisition
  • 08:51Results: Changes in Grb2 Localization by cDNA Expression
  • 10:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

高内涵筛选新的信号主管跨膜受体的鉴定方法。此方法是适合于大规模的自动化，并允许的预测<em在体内</em>蛋白结合和在哺乳动物细胞中的蛋白复合物的亚细胞定位。

Tags

High-content ImagingGrb2 Signaling ComplexesExpression CloningSignal TransductionGrowth Factor ReceptorsDownstream Signaling CascadesMitogenic SignalingSH2 DomainSH3 DomainGTPase Son-Of-Sevenless (SOS)Phosphorylated Tyrosine ResiduesSOS-Ras-MAP Kinase Signaling CascadePositive And Negative Regulator Of SignalingReceptor EndocytosisMicroscopy AssayPlasma Membrane ReceptorsEpidermal Growth Factor Receptor (EGFR)GFP-Grb2

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/4382?article_type=v

一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
check_url/it/4382?article_type=v

使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
check_url/it/4382?article_type=v

使用自动细胞计数器简化基因表达研究：在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
check_url/it/4382?article_type=v

石蜡包埋及冷冻的部分果蝇成人肌肉
check_url/it/4382?article_type=v

依赖的PDZ-CFTR的大分子信号复合物的体外分析
check_url/it/4382?article_type=v

肿瘤细胞球体的高通量图像分析：一个用户友好的软件应用来测量球体的大小自动准确
check_url/it/4382?article_type=v

代谢率的测量<em>果蝇</em>使用呼吸运动
check_url/it/4382?article_type=v

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
check_url/it/4382?article_type=v

工作流的高含量，荧光标记的万能工具显微镜数据，支持开源软件的单个细胞定量
check_url/it/4382?article_type=v

成像使用烟草瞬时表达系统MAPK信号通路的空间重组

Read Article