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高效率，站点特定的贴壁细胞转染siRNA的微电极阵列（MEA）

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Bioingegneria

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JoVE Journal Bioingegneria

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

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高效率，站点特定的贴壁细胞转染siRNA的微电极阵列（MEA）

DOI:

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09:14 min

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September 13, 2012

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Chetan Patel, Jit Muthuswamy

¹School of Biological and Health Systems Engineering,Arizona State University

Capitoli

00:05Titolo
01:26MEA Preparation and Cell Seeding
03:58Site-specific Transfection of siRNA and Electroporation
08:00Results: High Efficiency, Site-specific Transfection of Adherent Cells with siRNA using MEA
08:52Conclusion

Summary

Traduzione automatica

文章详细介绍了协议的站点特定的扰码序列的siRNA转染贴壁哺乳动物细胞培养用微电极阵列（MEA）。

Tags

SiRNA ShRNA RNAi Pathway Functional Genomics Therapeutics Delivery Techniques Electroporation Chemical Transfection Methods Cationic Lipids Cationic Polymers Nanoparticles High-throughput Technologies Single Cell Studies Cell-cell Interactions Gene Delivery Microscale Electroporation

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