È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:44Low Speed Extract Preparation
- 05:46Preparation of a DNA Damage-mimicking Structure (AT70)
- 06:31DNA Damage: MMS-treated Sperm Chromatin
- 07:35DNA Damage: UV-treated Sperm Chromatin
- 07:58Triggering the DNA Damage Checkpoint in LSE
- 08:54Induce DNA Damage Checkpoint in LSE with the AT70
- 09:35Results: DNA Damage Checkpoint of Xenopus Egg Extracts
- 10:05Conclusion
Xenopus egg extract is a useful model system to investigate the DNA damage checkpoint. This protocol is for the preparation of Xenopus egg extracts and DNA damage checkpoint inducing reagents. These techniques are adaptable to a variety of DNA damaging approaches in the study of the DNA damage checkpoint signaling.
Tags
DNA Damage CheckpointXenopus Egg ExtractsEndogenous InsultsEnvironmental InsultsDNA Damage Checkpoint SignalingSensorsTransducersEffectorsATR (ATM And Rad3-related)KinasesChk1 (Checkpoint Kinase 1)Cell Cycle ArrestTranscription ActivationDNA Damage RepairApoptosisSenescenceGenomic InstabilityGenomic IntegrityHuman DiseasesCancer DevelopmentXenopus Laevis Egg ExtractsCell-free Extract Model System
