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Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

JoVE Journal
Biologia

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JoVE Journal Biologia

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.

DOI:

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09:38 min

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October 12, 2006

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Erin Trish, John Dimos, Kevin Eggan

¹Department of Molecular and Cell Biology,Harvard

Capitoli

00:06ES cells in culture
00:28Preparation
02:06Trypsinize cells
04:46Separate ES cells and feeder cells
06:59Resuspend and split cells

Summary

Traduzione automatica

In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin. Brought to you by JoVE.

Tags

Biologia Trypsin Cell Culture Karyotypic Abnormalities Undifferentiated Culture Maintenance Trypsin-EDTA Cell Suspension HuES Media Split Ratio Re-plating Mouse Embryonic Fibroblast Feeder Cells Media Change

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