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Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Medicina
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JoVE Journal Medicina
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

DOI:
10.3791/50011-v

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13:08 min

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January 03, 2013

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, ,
1Life Science Division,Lawrence Berkeley National Laboratory

Capitoli

  • 00:05Titolo
  • 02:07Tissue Processing and Digestion
  • 04:53Filtration and Freezing of Digested Material
  • 07:02Subculture of Primary Cultures
  • 09:09Results: HMEC Organoids and Cultures
  • 12:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.

Tags

Human Mammary Epithelial CellsReduction MammoplastyMastectomyCell CultureOrganoidsEpithelial CellsMesenchymal CellsCell DifferentiationCell AgingCell ImmortalizationBreast Cancer

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