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In ovo Expression of MicroRNA in Ventral Chick Midbrain
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JoVE Journal Neuroscienze
In ovo Expression of MicroRNA in Ventral Chick Midbrain

In ovo Expression of MicroRNA in Ventral Chick Midbrain

DOI:
10.3791/50024-v

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09:19 min

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September 16, 2013

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, , , , , ,
1Department of Experimental Embryology, Institute of Anatomy,University of Tübingen

Capitoli

  • 00:00Titolo
  • 01:53Introduction
  • 02:23Finishing and filling of micropipettes
  • 03:00Incubation and preparation of eggs
  • 04:43DNA injection into midbrain/neural tube
  • 05:37Electroporation of midbrain
  • 07:06Removing, cleaning and fixing of embryo or fetus
  • 08:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Ectopic expression is one technique to elucidate the microRNAs role in brain development. However, targeting specific areas using in ovo electroporation is challenging. Here, we show an efficient way to selectively electroporate ventral and dorsal midbrain regions.

Tags

MicroRNAIn Ovo ElectroporationVentral MidbrainGene ExpressionNon-coding RNASpatial And Temporal ControlPlatinum ElectrodesTransfectionIn Vivo Studies

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