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Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
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JoVE Journal Bioingegneria
Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

DOI:
10.3791/50032-v

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06:37 min

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October 18, 2012

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, , , ,
1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Department of Pathology,New York University School of Medicine, 3Healthcare System,Veterans Affairs New York Harbor

Capitoli

  • 00:00Titolo
  • 00:15Introduction
  • 01:22Plasmid DNA Preparation
  • 02:10Tattoo Equipment Setup
  • 03:26Animal Skin Preparation
  • 04:14DNA Tattooing
  • 05:33Visualization of EGFP Expression
  • 06:04Conclusion

Summary

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Skin tattooing is a potent and safe way to delivery DNA vaccine intradermally. Here, a DNA plasmid encoding EGFP is delivered by tattooing to the skin of a laboratory mouse, and the expression of EGFP in the skin cells is then inspected by confocal microscopy.

Tags

Keywords: DNA VaccineNucleic Acid-based VaccinationPlasmid DNASkin TattooingIntradermal DeliveryEGFPConfocal MicroscopyAdjuvantCost-effectiveConvenient

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