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Capitoli
- 00:05Titolo
- 01:49Stage-specific Injection of Morpholinos (MO)
- 03:10Selecting Injected Embryos for Analysis
- 04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
- 05:56Visualization and Analysis of KV Fluid Flow
- 06:38Representative Kupffer’s Vesicle Fluid Flow Results
- 07:45Conclusion
Cilia-genereret fluidstrøm i Kupffer s vesikel (KV) styrer venstre-højre mønstret af den zebrafisk embryo. Her beskriver vi en teknik til at modulere genfunktion specifikt i KV celler. Derudover viser vi, hvordan man leverer fluorescerende perler i KV at visualisere fluidstrømning.
