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Immunologia e infezioni

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定量高通量单细胞的细胞毒性T细胞的测定

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

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定量高通量单细胞的细胞毒性T细胞的测定

DOI:

10.3791/50058-v

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09:28 min

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February 02, 2013

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Ivan Liadi, Jason Roszik, Gabrielle Romain, Laurence J.N. Cooper, Navin Varadarajan

¹Department of Chemical and Biomolecular Engineering,University of Houston , ²Division of Pediatrics, Research Unit 907,University of Texas MD Anderson Cancer Center

Capitoli

00:05Titolo
01:32Fabrication of Arrays of Nanowells in PDMS
02:14Target Cell (T) Labeling
02:53Effector (E) Cell Labeling
03:26Separation of Live and Dead Cells by Ficoll Density Gradient
05:23Cell Loading onto Nanowell Array
06:51Imaging
07:58Results: A High-throughput Cytolytic Assay
09:03Conclusion

Summary

Traduzione automatica

我们描述了一种单细胞的高通量的检测，以测量与肿瘤靶细胞的培养时的细胞毒性T细胞。此方法采用致密的，弹性体子纳升井（〜100,000井/数组）的数组，在空间上局限在T细胞和靶细胞，在规定的比例，和被耦合到的荧光显微镜来监测效靶共轭和随后的细胞凋亡。

Tags

Keywords: Single-cell Cytotoxicity Assay T Cells Cancer Immunotherapy Adoptive Cell Therapy CAR T Cells Cytotoxicity High-throughput Nanowell Array Cell-based Assay Time-lapse Microscopy

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