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C. elegans Chemotaxis Assay
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JoVE Journal Biologia
C. elegans Chemotaxis Assay

C. elegans Chemotaxis Assay

DOI:
10.3791/50069-v

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06:28 min

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April 27, 2013

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, ,
1Life Sciences,Queen’s University , 2Department of Chemical Engineering,Queen’s University , 3Department of Biology,Queen’s University

Capitoli

  • 00:05Titolo
  • 01:06Preparing the Test Plates
  • 01:45Preparing the Worms
  • 03:27Running the Assay
  • 05:29Comparing Wild-type (N2) C. elegans to the odr-10(ky10) Mutant
  • 05:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

A method of quantitatively evaluating the chemotactic response of Caenorhabditis elegans is described. A chemotactic index (CI) was employed as a way to precisely evaluate the response of worms to certain targets, and serve as a platform of comparison between strains and compounds of interest.

Tags

Keywords: C. ElegansChemotaxis AssayOdorant ResponseFour-quadrant SystemWorm BehaviorSample SizeOptimizationBargmann Et Al. Method

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