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结合免疫荧光和三维保存的间期核荧光原位杂交DNA研究在3D核组织的变化

JoVE Journal
Biologia

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JoVE Journal Biologia

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

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结合免疫荧光和三维保存的间期核荧光原位杂交DNA研究在3D核组织的变化

DOI:

10.3791/50087-v

•

13:55 min

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February 03, 2013

•

Julie Chaumeil, Mariann Micsinai^2,3,4, Jane A. Skok

¹Department of Pathology,New York University School of Medicine, ²New York University Center for Health Informatics and Bioinformatics, ³NYU Cancer Institute, ⁴Department of Pathology and Yale Cancer Center,Yale University School of Medicine

Capitoli

00:05Titolo
01:27DNA Probe Labeling with Fluorophores: Nick Translation
03:37Probe Precipitation/Denaturation/Pre-annealing
05:133-dimensional DNA FISH
09:253-dimensional DNA FISH / Immuno-FISH
10:40Combined Immunofluorescence and DNA FISH: Representative Results
12:56Conclusion

Summary

Traduzione automatica

在这里，我们描述了一个协议，用于同时检测组蛋白修饰免疫荧光法和DNA序列的DNA荧光原位杂交，然后通过的3D显微镜和分析（3D的免疫-DNA FISH）。

Tags

3D DNA FISH 3D Nuclear Organization 3D Confocal Microscopy Immunofluorescence Chromatin Modifications Gene Loci Chromosome Territories Nuclear Architecture Nuclear Proteins Histone Variants Transcription Machinery Nuclear Sub-compartments

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