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核糖体RNA的蚊子肠道宏基因组RNA-seq的枯竭
JoVE Journal
Biologia
Author Produced
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JoVE Journal Biologia
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
DOI:

06:21 min

April 07, 2013

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Capitoli

  • 00:02Titolo
  • 00:08Introduction
  • 00:48Mosquito Rearing
  • 01:31Mosquito gut dissection
  • 01:43Metagenomic DNA and RNA isolation
  • 02:27Ribosomal RNA depletion
  • 02:34PCR amplification of rRNA gene fragments
  • 02:48In vitro transcription of rRNA probes
  • 03:19rRNA subtraction
  • 03:39Removal of rRNA
  • 05:07Representative results
  • 05:48Conclusion

Summary

Traduzione automatica

核糖体RNA(rRNA)的消耗协议的开发是为了丰富信使RNA(mRNA),RNA-seq中蚊子的肠道metatranscriptome的。由蚊子和其肠道微生物样品的具体rRNA探针,用于去除rRNA基因通过减法,创建了。协议的性能的影响,可能会导致在约90-99%的rRNA基因的去除。

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