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电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚
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Neuroscienze
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JoVE Journal Neuroscienze
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚

DOI:
10.3791/50136-v

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10:04 min

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May 29, 2013

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, , ,
1Koret School of Veterinary Medicine,The Hebrew University of Jerusalem, 2Department of Medical Neurobiology,The Hebrew University of Jerusalem

Capitoli

  • 00:05Titolo
  • 01:17Egg Handling and Preparation
  • 02:18Windowing, Injection and Electroporation
  • 04:22Analysis of Embryos
  • 06:18Cryo-sections and Immunofluorescence
  • 07:14Representative Electroporation Results
  • 09:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

神经网络是建立在胚胎大脑发育神经生物学的一个基本问题。在这里，我们结合电穿孔技术与新的遗传工具，如酶Cre / LOX-质粒和PiggyBac转介导的DNA换位系统在禽流后脑背中间贴上标签，并跟踪他们的轴突预测和突触在不同发育阶段的目标。

Tags

ElectroporationHindbrainChick EmbryoAxonal TrajectorySynaptic TargetNeural TubeCre/LoxPiggyBacGFPInterneuronsDA1Brainstem

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