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Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
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JoVE Journal Bioingegneria
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

DOI:
10.3791/50143-v

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10:41 min

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August 20, 2013

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, , , ,
1Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute,University of Groningen, 2Department for the Biology of Ageing, European Research Institute for the Biology of Ageing,University Medical Centre Groningen, University of Groningen, 3Institute of Biochemistry,ETH Zurich, 4Department of Health Sciences and Technology,ETH Zurich, 5Institute of Molecular Systems Biology,ETH Zurich

Capitoli

  • 00:05Titolo
  • 01:32Production and Preparation of a Silicon Wafer Mold
  • 02:14Production of Microfluidic Chips
  • 05:10Preparing the Chip for Cell Loading
  • 06:20Loading of Yeast Cells into the Microfluidic Chip
  • 07:53Results: Observation of Replicative Aging of Budding Yeast
  • 10:04Conclusion

Summary

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We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan.

Tags

Keywords: Microfluidic ChipSingle Cell TrackingBudding YeastReplicative AgingCell RetentionHigh-resolution Microscopy

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