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Mærkning af enkeltceller i centralnervesystemet hos<em> Drosophila melanogaster</em
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Neuroscienze
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JoVE Journal Neuroscienze
Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Mærkning af enkeltceller i centralnervesystemet hos<em> Drosophila melanogaster</em

DOI:
10.3791/50150-v

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10:33 min

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March 04, 2013

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, , , ,
1Institute of Genetics,University of Mainz, 2Department of Anatomy and Neuroscience,University of Melbourne

Capitoli

  • 00:05Titolo
  • 01:14Embryo Collection and Preparation
  • 04:21Dye Injection of Individual Neruons
  • 08:26DiI Labeled Neurons
  • 10:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi præsenterer en teknik til mærkning af enkelte neuroner i centralnervesystemet (CNS) af<em> Drosophila</em> Embryoner, som giver analyse af neuronal morfologi ved enten lystransmission eller konfokal mikroskopi.

Tags

Keywords: Single Cell LabelingDrosophila MelanogasterCentral Nervous SystemDiIJuxtacellular InjectionNeuronal Cell MorphologyPhotoconversionConfocal MicroscopyGenetically Encoded Fluorescent MarkersMutant Phenotypes

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