Rivista
/
Biologia
/
Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

DOI:
10.3791/50151-v

•

08:59 min

•

August 18, 2013

•

, ,
1Department of Anatomy and Cell Biology and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine,Technion-Israel Institute of Technology

Capitoli

  • 00:05Titolo
  • 01:38Preparing Coverslips for Embryo Collection
  • 02:32Embryo Collection and Preparation
  • 05:38Embryo Injection
  • 06:40Imaging the Injected Embryos
  • 07:28Annexin V Injection Results
  • 08:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe an effective method for studying dynamics of apoptotic cell clearance in vivo. This method employs live Drosophila embryos as a powerful model for monitoring phagocytosis of apoptotic cells using specific labeling of apoptotic cells and phagocytes.

Tags

Apoptotic Cell ClearancePhagocytosisDrosophila EmbryogenesisLive ImagingMacrophagesGliaCentral Nervous SystemCell Death

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

Preparing Individual Drosophila Egg Chambers for Live Imaging

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

4D Imaging of Protein Aggregation in Live Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Read Article