/

/

鉴定<em>睡美人</em>转座子插入连接器介导的PCR实体瘤

JoVE Journal
Medicina

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicina

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Please note that all translations are automatically generated. Click here for the English version.

鉴定<em>睡美人</em>转座子插入连接器介导的PCR实体瘤

DOI:

10.3791/50156-v

•

10:34 min

•

February 01, 2013

•

Callie L. Janik², Timothy K. Starr²

¹Department of Obstetrics, Gynecology & Women’s Health, Masonic Cancer Center,University of Minnesota, Minneapolis, ²Department of Genetics, Cell Biology & Development, Center for Genome Engineering,University of Minnesota, Minneapolis

Capitoli

00:05Titolo
01:00Tumor Collection and DNA Isolation
02:16Digesting and Ligating Linkers to the DNA
05:51Removing Concatamer Transposons Using a Second Digestion
06:54Amplifying the Tumor DNA
09:19PCR and Sequencing Results
10:08Conclusion

Summary

Traduzione automatica

鉴定未知的驱动程序致癌作用的一种方法，用一种公正的方法。该方法使用<em>睡美人</em>转座子作为随机诱变剂，向特定的组织。肿瘤的形成转座子插入驱动的新型癌基因和肿瘤抑制基因的基因组作图

Tags

Sleeping Beauty Transposon Forward Genetic Screen Linker-mediated PCR Tumor Genetics Cancer Genome Genetic Drivers Passenger Mutations Genetic Heterogeneity Cre Recombinase Transposase Common Insertion Sites

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

在免疫缺陷小鼠的建立和传播人类视网膜母细胞瘤肿瘤

使用实时活体成像在肿瘤纳米粒子吸收的评价

在使用定量免疫肿瘤的异质性蛋白表达的映射

实时定量PCR（qPCR的microRNA检测在前列腺肿瘤）

在体外化培养基层小鼠结肠肿瘤

脑脊液的microRNA分析采用实时定量PCR技术

在脑肿瘤和关节炎的用荧光SAPC-DOPS纳米囊泡体光学成像

对源自液体和固体肿瘤癌症细胞定量蛋白质组质谱剖析

灌注的空间测量，实体肿瘤间质流体压力和脂质体积累

神经外科复杂颅内肿瘤的多色3D打印

Read Article