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视黄醇运输的血浆视黄醇结合蛋白的膜受体的实时分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

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视黄醇运输的血浆视黄醇结合蛋白的膜受体的实时分析

DOI:

10.3791/50169-v

•

14:32 min

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January 28, 2013

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Riki Kawaguchi, Ming Zhong, Hui Sun

¹Department of Physiology, Jules Stein Eye Institute and Howard Hughes Medical Institute,University of California, Los Angeles

Capitoli

00:05Titolo
01:50Production, Refolding, and HPLC Purification of holo-RBP
07:45Real-time Monitoring of STRA6-catalyzed Retinol Release and Retinol Loading
10:16Real-time Monitoring of STRA6-catalyzed Transport of Retinol from holo-RBP to CRBP-I
12:40Representative Data of Real-time Analysis of STRA6-catalyzed Retinol Transport
13:47Conclusion

Summary

Traduzione automatica

在这里，我们描述了一个优化的技术，生产高品质的维生素A / RBP复杂的和实时监测技术研究维生素A运输STRA6，RBP受体。

Tags

Retinol Transport Plasma Retinol Binding Protein Holo-RBP STRA6 Real-time Analysis Electrophysiology CRBP-I Vitamin A Transport Mechanism

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