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原位膀胱癌模型的基因传递研究
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Medicina
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JoVE Journal Medicina
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
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原位膀胱癌模型的基因传递研究

DOI:
10.3791/50181-v

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07:48 min

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December 01, 2013

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1Department of Microbiology & Immunology,Medical University of South Carolina

Capitoli

  • 00:05Titolo
  • 01:01Cell Implantation
  • 03:19Preparing MB49 Cells and Implantation
  • 05:00Intravesical Delivery of Adenovirus
  • 06:27Representative Bladder Cancer Initiation Results
  • 07:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

植入癌细胞进入器官来源可以作为一个有用的临床前模型来评估新疗法。 MB49膀胱癌细胞可在膀胱内以下膀胱灌注生长。该协议表明了鼠标膀胱肿瘤植入和腺病毒转染的目的导尿。

Tags

Keywords: Orthotopic Bladder Cancer ModelMB49 Murine Bladder Carcinoma CellsCatheterizationGlycosaminoglycan LayerTrypsinAdenoviral ConstructLuciferase Reporter GeneGene Delivery Studies

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