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Biology

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Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
 

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

Article DOI: 10.3791/50185-v 07:39 min May 3rd, 2013
May 3rd, 2013

Capitoli

Riepilogo

We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.

Tags

Keywords: Adult Stem Cells SKPs Skin-derived Precursors Primary Fibroblast Cultures Multipotent Precursor Cells Dermal Precursors Nestin Vimentin Fibronectin Stem Cell Markers Tissue Banks Genetic Diseases Disease Modeling
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