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Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
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Capitoli
- 00:05Titolo
- 01:35Electrode Preparation
- 02:14Ganglia and Muscle Preparation
- 07:42Setting up the Extracellular Glass Electrodes for Soma Recordings
- 08:35Setting up the Suction Electrodes for the Nerve Recordings
- 09:41Setting up the Force Transducer to Measure the I1/I3 Muscle Contraction
- 10:38Results: Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
- 13:19Conclusion
In animals with large identified neurons (e.g. mollusks), analysis of motor pools is done using intracellular techniques1,2,3,4. Recently, we developed a technique to extracellularly stimulate and record individual neurons in Aplysia californica5. We now describe a protocol for using this technique to uniquely identify and characterize motor neurons within a motor pool.
