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1Edinburgh Urological Cancer Group,University of Edinburgh, 2School of Medicine,University of St Andrews, 3Division of Pathology,University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM,University of Edinburgh, 5Department of Pathology,Western General Hospital, 6Breakthrough Breast Cancer Research Unit,University of Edinburgh, 7St Bartholomew’s Cancer Institute, Experimental Cancer Medicine Centre,Queen Mary University of London
Capitoli
- 00:05Titolo
- 01:39Identification of Morphological and Molecular Tumor Heterogeneity
- 02:58Protein Extraction from Tumor Samples
- 04:24RPPA Printing
- 06:00RPPA Protein Detection
- 09:27Results: Pre- and Post-TKI-treated Renal Cell Cancer Show Differential Protein Expression
- 12:06Conclusion
RPPA muliggør protein ekspression af hundreder af prøver, udskrives på nitrocellulose objektglas til at blive udspurgt samtidigt under anvendelse af fluorescensmærkede antistoffer. Denne teknik er blevet anvendt til at studere virkningen af lægemiddelbehandling heterogenitet inden clear cell renal carcinoma.
