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巨脂质体制剂成像和膜片钳电
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Biologia
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JoVE Journal Biologia
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
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巨脂质体制剂成像和膜片钳电

DOI:
10.3791/50227-v

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09:03 min

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June 21, 2013

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,
1Department of Physiology and Biophysics,University of Washington

Capitoli

  • 00:05Titolo
  • 01:19Deposition of Lipids from Organic Solvents: Classical Protocol
  • 03:13Electroformation of Giant Liposomes
  • 05:19Deposition of Lipids by Controlled Dehydration of Small Liposomes
  • 07:10Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
  • 08:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

重构功能膜蛋白组成界定成巨脂质体是一个功能强大的方法，结合膜片钳电。然而，传统的巨头脂质体产量可能与蛋白质的稳定性不相容。我们描述生产从单纯的脂质的巨脂质体或小脂质体含有离子通道的协议。

Tags

Giant LiposomesIon ChannelsElectrophysiologyPatch-clampElectroformationLipid MembranesReconstitutionSmall LiposomesDehydrationVapor EquilibriumSaturated Salt Solutions

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