Rivista
/
Biologia
/
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

DOI:
10.3791/50246-v

•

12:08 min

•

May 22, 2013

•

, ,
1Department of Medicine,Albert Einstein College of Medicine , 2Diabetes Research and Training Center,Albert Einstein College of Medicine , 3Department of Genetics,Albert Einstein College of Medicine

Capitoli

  • 00:05Titolo
  • 01:49Telomere Length Measurement using Quantitative Real-Time PCR
  • 05:50Quantitative Telomerase Detection Activity Measurement
  • 09:47Results: Quantitative Real-Time PCR is Used to Determine Telomere length and Telomerase Activity in Human Leukocytes
  • 11:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

An accurate, short, sophisticated and cheap method is described that assesses telomere length in multiple tissues and species using qRT-PCR. In addition, we will describe a simple assay to assess telomerase activity as a complementary backbone test for telomere length.

Tags

Keywords: Telomere LengthTelomerase ActivityCell SenescenceChromosome AttritionCell DivisionCell AgingQRT-PCRTelomere SequenceTelomere AssayTelomerase Assay

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/50246?article_type=v

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
check_url/it/50246?article_type=v

In vitro Reconstitution of the Active T. castaneum Telomerase
check_url/it/50246?article_type=v

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/it/50246?article_type=v

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
check_url/it/50246?article_type=v

Measurement of Cellular Chemotaxis with ECIS/Taxis
check_url/it/50246?article_type=v

Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
check_url/it/50246?article_type=v

In vitro Cell Migration and Invasion Assays
check_url/it/50246?article_type=v

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
check_url/it/50246?article_type=v

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
check_url/it/50246?article_type=v

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Read Article