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分离的小鼠胚胎面外胚层和间充质
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Biologia
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JoVE Journal Biologia
Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
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分离的小鼠胚胎面外胚层和间充质

DOI:
10.3791/50248-v

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08:36 min

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April 12, 2013

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,
1Department of Craniofacial Biology,University of Colorado Denver Anschutz Medical Campus, 2Department of Cell and Developmental Biology,University of Colorado Denver Anschutz Medical Campus

Capitoli

  • 00:05Titolo
  • 01:34Dissecting CD-1 E10.5 Embryos
  • 03:18Dispase II Treatment
  • 04:13Separating the Facial Ectoderm and Mesenchyme
  • 06:37Tissue Purity after Separation
  • 07:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

外胚层和间充质细胞的胚胎面部分离的协议。我们使用的是中性蛋白酶II对待整个胚胎首先，剖析整个面部突起，然后分离的面部外胚层和间充质细胞。

Tags

Facial EctodermFacial MesenchymeOrofacial CleftsFacial ProminencesEmbryonic DevelopmentMouse ModelDispase IIGene ExpressionTranscription FactorsChromatin Marks

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