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代高通量测序技术（ChIP-seq的高品质的染色质免疫沉淀DNA模板）

JoVE Journal
Biologia

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JoVE Journal Biologia

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

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代高通量测序技术（ChIP-seq的高品质的染色质免疫沉淀DNA模板）

DOI:

10.3791/50286-v

•

09:52 min

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April 19, 2013

•

Sandra Deliard, Jianhua Zhao, Qianghua Xia, Struan F.A. Grant²

¹Division of Human Genetics,Children’s Hospital of Philadelphia Research Institute, ²Department of Pediatrics, Perelman School of Medicine,University of Pennsylvania

Capitoli

00:05Titolo
00:59Cross-linking Chromatin
02:27Sonication
04:14Pre-clearing Chromatin
05:15Chromatin Immunoprecipitation
07:10Reversing Cross-link and Purifying DNA
08:12Results: Generation of a High Quality ChIP Template
09:28Conclusion

Summary

Traduzione automatica

染色质免疫沉淀和超高通量测序（芯片起）的组合可以识别并映射在一个给定的组织或细胞系的蛋白质-DNA相互作用。概述是如何产生一个高质量的ChIP随后的测序模板，使用的经验，例如转录因子TCF7L2。

Tags

Keywords: ChIP-seq Chromatin Immunoprecipitation High-throughput Sequencing Transcription Factors TCF7L2 Type 2 Diabetes Cancer HCT116 Protein-DNA Interactions Genome Sequencing

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