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单个锥体神经元的高保真光遗传学控制方法<em>在体内</em
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
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单个锥体神经元的高保真光遗传学控制方法<em>在体内</em

DOI:
10.3791/50291-v

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13:44 min

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September 02, 2013

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, ,
1Institute for Behavioral Genetics,University of Colorado Boulder, 2Department of Psychology and Neuroscience,University of Colorado Boulder

Capitoli

  • 00:05Titolo
  • 01:50Virus Storage and Preparation
  • 02:57Surgery and Virus Injection
  • 04:59Light Delivery and In vivo Recording of CHR2 or NpHR-Expressing Neurons
  • 08:38Results: High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
  • 13:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

神经科学的工具，结合遗传学和光学的最新发展，被称为“光遗传学”，使过度的神经回路活动控制与空间和时间分辨率的一个前所未有的高度。在这里，我们提供了一个协议，用于在体内录音与集成光遗传学操作的前额叶皮层和subicular锥体神经元的基因定义的子集。

Tags

Keywords: OptogeneticsNeural CircuitChannelrhodopsin-2HalorhodopsinAdeno-associated VirusDorsal SubiculumPrelimbic RegionPrefrontal CortexPyramidal NeuronIn VivoElectrical ActivityLight DeliveryElectrical Recording

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