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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
 

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Article DOI: 10.3791/50294-v • 10:45 min • June 14th, 2013
June 14th, 2013 •
1, 1, 1
1Department of Pharmacology & Physiology, Drexel University College of Medicine

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The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

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Purification MicroRNA Profiling Exosomes Blood Culture Media Stable MiRNAs Body Fluids Circulating MiRNAs Degradation Sequestration Small Vesicles Fusion Transfer Of RNA And Proteins Biological Functions Immune Response Antigen Presentation Intracellular Communication Delivery Of MiRNAs Target Intervention Drugs RNA Therapeutic Agents Biomarkers Diagnosis Treatment Options Prognosis Quantitatively Analyzing MiRNAs And MRNA Exosomes Secreted In Blood And Cell Culture Media Western Blot Analysis PCR Exosomal Markers Transmission Electron Microscopy (TEM) Immunogold Labeling Exosomal Morphology And Integrity Validation Total RNA Purification Bioanalyzer Validation Of RNA Integrity Quantitative Real-time PCR (qPCR) Exosomal MiRNA Identification
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