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听话的哺乳动物细胞原生动物引发的细菌感染
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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听话的哺乳动物细胞原生动物引发的细菌感染

DOI:
10.3791/50300-v

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13:54 min

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April 02, 2013

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, , ,
1Department of Molecular Microbiology & Immunology,Oregon Health & Science University

Capitoli

  • 00:05Titolo
  • 02:13Preparation of Legionella pneumophila Cultures for Priming Stage Infections
  • 04:14Priming Stage Infection Using Acanthamoeba castellanii
  • 07:13Processing of Bacteria for Target Cell Stage Infection after the Priming Stage Infection
  • 09:46Experimental Analysis
  • 11:14CFU Plating
  • 11:51Representative Infection Results
  • 13:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

这种技术提供了一种方法来收集，标准化和量化，自然原虫的宿主细胞培养的哺乳动物细胞感染前细胞内生长的细菌病原体。此方法可以被修改，以适应各种各样的起动阶段，以及靶细胞类型的宿主细胞。

Tags

Keywords: TractableMammalian Cell InfectionsProtozoan-primed BacteriaIntracellular Bacterial PathogensLegionella PneumophilaLegionnaires’ PneumoniaAcanthamoeba CastellaniiVirulence FactorsGFP-expressing StrainsFluorescence MicroscopyFlow CytometryColony Plating

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