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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
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JoVE Journal Bioingegneria
Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

DOI:
10.3791/50310-v

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13:10 min

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April 04, 2013

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, , , , , , ,
1Centre for Vascular Research and Australian Centre for Nanomedicine,The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine,The University of New South Wales

Capitoli

  • 00:05Titolo
  • 02:17Passivation of Glass Coverslips with PLL-PEG-biotin
  • 04:04Fabrication of Stamps for Microcontact Printing
  • 04:38Patterning Streptavidin or Adhesive Cues onto Passivated Glass Coverslips
  • 06:13Creating Adhesive Gradients onto Streptavidin-pattern Surfaces
  • 07:47Loading of Cells with a Soluble Gradient into the Microfluidics Device for Live Cell Imaging
  • 09:08Results: Cell Migration in Response to Adhesive and Soluble Gradients
  • 12:16Conclusion

Summary

Traduzione automatica

Traduzione automatica

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

Tags

Adhesive GradientSoluble GradientCell MigrationFluorescence MicroscopyMicrofluidicsPLL-PEGStreptavidinRGD PeptideChemoattractantFBS

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