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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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JoVE Journal Neuroscienze
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

DOI:
10.3791/50312-v

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07:45 min

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April 30, 2013

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,
1Department of Physiology,University of California, Los Angeles

Capitoli

  • 00:05Titolo
  • 01:27Dissection of Brains from Adult Medaka
  • 02:56Mounting the Brain in a Recording Chamber
  • 03:37Electrophysiology: Loose Patch Recording of Action Potential Firing
  • 05:36Electrophysiology: Whole Cell Recording of Membrane Potential
  • 06:26GnRH3 Neuron Activity in Medaka and Zebrafish
  • 07:27Conclusion

Summary

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In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.

Tags

Transgenic FishGnRH NeuronsMedaka FishTerminal NerveNeuromodulatorySpontaneous Action Potential FiringKisspeptinIntact BrainNeuron IdentificationElectrophysiology

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